PLD1 antibody (pThr147)

Catalog No. ABIN7138779

The Rabbit Polyclonal anti-PLD1 antibody is suitable to detect PLD1 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for PLD1 antibody (pThr147) (ABIN7138779)

Target: PLD1 (Phospholipase D1 (PLD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLD1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-PLD1 Antibody

Binding Specificity: pThr147

Purpose: Phospho-PLD1 (Thr147) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PLD1

Target: PLD1 (Phospholipase D1 (PLD1))

Alternative Name: PLD1

Background:

Background:

Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

Aliases: Choline phosphatase 1 antibody, hPLD1 antibody, Phosphatidylcholine-hydrolyzing phospholipase D1 antibody, Phospholipase D1 antibody, PLD 1 antibody, PLD1 antibody, PLD1_HUMAN antibody

UniProt: Q13393