Caveolin-1 antibody (pTyr14)

Catalog No. ABIN7138938

The Rabbit Polyclonal anti-Caveolin-1 antibody has been validated for WB, ELISA and IF. It is suitable to detect Caveolin-1 in samples from Human.

Quick Overview for Caveolin-1 antibody (pTyr14) (ABIN7138938)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Caveolin-1 Antibody

Binding Specificity: pTyr14

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 14 (H-L-Y(p)-T-V) derived from Human CAVEOLIN-1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1

Background:

Background: The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.

Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250.
Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140.
Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586.
Labrecque L, et al. (2003) Mol Biol Cell. 14(1): 334-347.
Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.

Aliases: BSCL3 antibody, CAV antibody, CAV1 antibody, CAV1_HUMAN antibody, caveolae protein, 22 kD antibody, caveolin 1 alpha isoform antibody, caveolin 1 beta isoform antibody, Caveolin 1 caveolae protein 22 kDa antibody, Caveolin-1 antibody, Caveolin1 antibody, cell growth-inhibiting protein 32 antibody, CGL3 antibody, LCCNS antibody, MSTP085 antibody, OTTHUMP00000025031 antibody, PPH3 antibody, VIP 21 antibody, VIP21 antibody

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals