Vinculin antibody (pTyr822)

Catalog No. ABIN7139077

This anti-Vinculin antibody is a Rabbit Polyclonal antibody detecting Vinculin in WB and ELISA. Suitable for Human.

Quick Overview for Vinculin antibody (pTyr822) (ABIN7139077)

Target: Vinculin (VCL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Vinculin Antibody

Binding Specificity: pTyr822

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL

Background:

Background:

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Subauste MC, et al. (2004) J Cell Biol 165, 371-81
Olsen JV, et al. (2010) Sci Signal 3, ra3
J?rgensen C, et al. (2009) Science 326, 1502-9

Aliases: CMD1W antibody, CMH15 antibody, Epididymis luminal protein 114 antibody, HEL114 antibody, Metavinculin antibody, MV antibody, MVCL antibody, OTTHUMP00000019861 antibody, OTTHUMP00000019862 antibody, VCL antibody, VINC antibody, VINC_HUMAN antibody, Vinculin antibody

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2