African Cassava Mosaic Virus AC1 (ACMV AC1) (AA 1-358) antibody (HRP)

Catalog No. ABIN7140392

This Rabbit Polyclonal antibody specifically detects African Cassava Mosaic Virus AC1 in ELISA. It exhibits reactivity toward African cassava mosaic virus (ACMV).

Quick Overview for African Cassava Mosaic Virus AC1 (ACMV AC1) (AA 1-358) antibody (HRP) (ABIN7140392)

Target: African Cassava Mosaic Virus AC1 (ACMV AC1)

Reactivity: African cassava mosaic virus (ACMV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Product Details

Binding Specificity: AA 1-358

Virus Strain: isolate West Kenyan 844

Purpose: AC1 Antibody, HRP conjugated

Cross-Reactivity: African cassava mosaic virus (ACMV)

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant African cassava mosaic virus Replication-associated protein (1-358AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: African Cassava Mosaic Virus AC1 (ACMV AC1)

Alternative Name: African cassava mosaic virus (isolate West Kenyan 844) (ACMV) (Cassava latent virus (isolate West Kenyan 844)) AC1

Background:

Background: Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5\'-TAATATTAC-3\' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5\'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3\'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).

Aliases: AC1 antibody, AL1 antibody, Replication-associated protein antibody, Rep antibody, EC 2.7.7.- antibody, EC 3.1.21.- antibody, 40.4 kDa protein antibody, Protein AC1 antibody, Protein AL1 antibody

UniProt: P14982