Tir (AA 252-362) antibody (HRP)

Catalog No. ABIN7141629

The Rabbit Polyclonal anti-Tir antibody is suitable to detect Tir in samples from E. coli. It has been validated for ELISA.

Quick Overview for Tir (AA 252-362) antibody (HRP) (ABIN7141629)

Target: Tir

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Product Details

Binding Specificity: AA 252-362

Purpose: tir Antibody, HRP conjugated

Cross-Reactivity: E. coli

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant Escherichia coli O157:H7 Translocated intimin receptor Tir protein (252-362AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: Tir

Background:

Background: Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion (By similarity).

Aliases: tir antibody, espE antibody, ECSP_4676 antibody, Translocated intimin receptor Tir antibody, Secreted effector protein Tir antibody

UniProt: C6UYL8