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ENO1 antibody (AA 2-434)

Name: ENO1 antibody (AA 2-434)
Brand: antibodies-online
SKU: ABIN7143869
Price: 462 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects ENO1 in WB, IHC, ELISA, IF, IP and FACS. It exhibits reactivity toward Human.

Western Blot Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0. (ENO1 antibody (AA 2-434))

IHC image of ABIN7143869 diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. (ENO1 antibody (AA 2-434))

IHC

Western Blot Positive WB detected in: HepG2 whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (ENO1 antibody (AA 2-434))

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s (ENO1 antibody (AA 2-434))

Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0. (ENO1 antibody (AA 2-434))

IHC image of ABIN7143869 diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. (ENO1 antibody (AA 2-434))

IHC

IHC image of ABIN7143869 diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. (ENO1 antibody (AA 2-434))

IHC

9 images

Catalog No. ABIN7143869

Quick Overview for ENO1 antibody (AA 2-434) (ABIN7143869)

Target

See all ENO1 Antibodies

ENO1 (Enolase 1 (ENO1))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This ENO1 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone

8H9G12

Discover our top product ENO1 Primary Antibody

Binding Specificity
21

7

6

6

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 2-434

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Purification

Protein G purified

Immunogen

Recombinant Human Alpha-enolase protein (2-434AA)

Isotype

IgG1, IgG1 kappa
anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA, IF, IP, IHC (p) Host: Mouse Monoclonal 8G8 unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) antibody
ENO1 Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (Middle Region) antibody
ENO1 Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Rabbit, Horse, Dog, Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 405-434), (C-Term) antibody
ENO1 Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB19995 unconjugated

anti-Enolase 1 (ENO1) (AA 246-295) antibody
ENO1 Reactivity: Human, Horse, Monkey, Pig, Chicken WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 8G3 unconjugated

anti-Enolase 1 (ENO1) antibody
KO Validated ENO1 Reactivity: Human WB, IF, ICC, IHC (p), DB, IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 2-428) antibody
ENO1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB: 1:5000-1:320000, IHC:1:200-1:500, IF:1:50-1:200, FC:1:100-1:300, IP:1 μL-4 μL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

ENO1 (Enolase 1 (ENO1))

Alternative Name

ENO1

Background

Background: ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Aliases: Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) ( NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1, MBPB1, MPB1

UniProt

P06733