Angiopoietin 1 antibody (AA 16-498)

Catalog No. ABIN7144088

This Rabbit Polyclonal antibody specifically detects Angiopoietin 1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Angiopoietin 1 antibody (AA 16-498) (ABIN7144088)

Target: Angiopoietin 1 (ANGPT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Angiopoietin 1 Antibody

Binding Specificity: AA 16-498

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Angiopoietin-1 protein (16-498AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1

Background:

Background: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Aliases: AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG antibody, ANG-1 antibody, ANG1 antibody, Angiopoietin-1 antibody, Angiopoietin1 antibody, ANGP1_HUMAN antibody, ANGPT 1 antibody, Angpt1 antibody, KIAA0003 antibody, OTTHUMP00000227592 antibody, OTTHUMP00000227599 antibody, OTTHUMP00000227600 antibody

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process