MAP4K4 antibody (pSer629) (Cy3)

Catalog No. ABIN710354

The Rabbit Polyclonal anti-MAP4K4 antibody is suitable to detect MAP4K4 in samples from Human. It has been validated for IF (cc) and IF (p).

Quick Overview for MAP4K4 antibody (pSer629) (Cy3) (ABIN710354)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MAP4K4 Antibody (Cy3)

Binding Specificity: pSer629

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MAP4K4 around the phosphorylation site of Ser629

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4

Background:

Synonyms: MAP4K4phospho S629, Hepatocyte progenitor kinase like/germinal center kinase like kinase, HGK, HPK/GCK like kinase, HPK/GCK like kinase HGK, KIAA0687, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, MEKKK4, Mitogen activated protein kinase kinase kinase kinase 4, Nck interacting kinase, NIK.

Background: MAP4K4 (Mitogen-activated protein kinase kinase kinase kinase 4) belongs to the serine/threonine protein kinase family and specifically activates MAPK8/JNK. It may play a role in the response to environmental stress and is thought to function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF alpha signaling pathway. It interacts with the SH3 domain of the adapter protein Nck. MAP4K4-dependent signaling inhibits PPARG responsive gene expression, adipogenesis, and insulin stimulated glucose transport.

Gene ID: 9448