CHMP4B antibody (AA 1-224)

Catalog No. ABIN7147524

The Rabbit Polyclonal anti-CHMP4B antibody has been validated for IHC and ELISA. It is suitable to detect CHMP4B in samples from Human.

Quick Overview for CHMP4B antibody (AA 1-224) (ABIN7147524)

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP4B antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-CHMP4B Antibody

Binding Specificity: AA 1-224

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Charged multivesicular body protein 4b protein (1-224AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CHMP4B

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Alternative Name: CHMP4B

Background:

Background: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released (PubMed:12860994, PubMed:18209100). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Aliases: Charged multivesicular body protein 4b antibody, CHM4B_HUMAN antibody, chmp4b antibody, Chromatin-modifying protein 4b antibody, hSnf7-2 antibody, hVps32-2 antibody, SNF7 homolog associated with Alix 1 antibody, SNF7-2 antibody, Vacuolar protein sorting-associated protein 32-2 antibody, Vps32-2 antibody

UniProt: Q9H444