EIF2AK1 antibody (AA 1-86)

Catalog No. ABIN7152137

This Rabbit Polyclonal antibody specifically detects EIF2AK1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for EIF2AK1 antibody (AA 1-86) (ABIN7152137)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF2AK1 Antibody

Binding Specificity: AA 1-86

Cross-Reactivity: Human, Rat

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Eukaryotic translation initiation factor 2-alpha kinase 1 protein (1-86AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: EIF2AK1

Background:

Background: Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at \\\'Ser-48\\\' and \\\'Ser-51\\\', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).

Aliases: E2AK1_HUMAN antibody, EC 2.7.11.1 antibody, Eif2ak1 antibody, Eukaryotic translation initiation factor 2 alpha kinase 1 antibody, Eukaryotic translation initiation factor 2-alpha kinase 1 antibody, HCR antibody, Heme controlled repressor antibody, Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody, Heme regulated inhibitor antibody, Heme regulated initiation factor 2 alpha kinase antibody, Heme regulated repressor antibody, Heme sensitive initiation factor 2a kinase antibody, Heme-controlled repressor antibody, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody, Heme-regulated inhibitor antibody, Hemin sensitive initiation factor 2 alpha kinase antibody, Hemin-sensitive initiation factor 2-alpha kinase antibody, HRI antibody, KIAA1369 antibody, PRO1362 antibody

UniProt: Q9BQI3

Pathways: Hepatitis C