GRK5 antibody (AA 406-590) (Biotin)

Catalog No. ABIN7153298

This Rabbit Polyclonal antibody specifically detects GRK5 in ELISA. It exhibits reactivity toward Human.

Quick Overview for GRK5 antibody (AA 406-590) (Biotin) (ABIN7153298)

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK5 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-GRK5 Antibody (Biotin)

Binding Specificity: AA 406-590

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human G protein-coupled receptor kinase 5 protein (406-590AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for GRK5

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Alternative Name: GRK5

Background:

Background: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).

Aliases: FLJ39780 antibody, FP2025 antibody, G protein coupled receptor kinase 5 antibody, G protein coupled receptor kinase GRK5 antibody, G protein-coupled receptor kinase 5 antibody, G protein-coupled receptor kinase GRK5 antibody, GPRK5 antibody, GRK-pan antibody, GRK5 antibody, GRK5_HUMAN antibody, Pan-GRK antibody

UniProt: P34947

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling