Nanog antibody (AA 1-305)

Catalog No. ABIN7155816

The Mouse Monoclonal anti-Nanog antibody has been validated for WB, ELISA, IF, FACS and ICC. It is suitable to detect Nanog in samples from Human.

Quick Overview for Nanog antibody (AA 1-305) (ABIN7155816)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 8A1D11

Product Details anti-Nanog Antibody

Binding Specificity: AA 1-305

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G purified

Immunogen: Recombinant Human Homeobox protein NANOG protein (1-305AA)

Isotype: IgG1, IgG1 kappa

Application Details

Application Notes: Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background:

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Aliases: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance