14-3-3 theta antibody (pThr232) (HRP)

Catalog No. ABIN711830

The Rabbit Polyclonal anti-14-3-3 theta antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect 14-3-3 theta in samples from Human.

Quick Overview for 14-3-3 theta antibody (pThr232) (HRP) (ABIN711830)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-14-3-3 theta Antibody (HRP)

Binding Specificity: pThr232

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human 14-3-3 around the phosphorylation site of Thr232

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3

Background:

Synonyms: 14-3-3, mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, tyrosine 3/tryptophan 5 -monooxygenase activation protein.

Background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Gene ID: 7531

Pathways: Apoptosis, Myometrial Relaxation and Contraction