NCBP2 antibody (AA 1-156)

Catalog No. ABIN7161752

This Rabbit Polyclonal antibody specifically detects NCBP2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for NCBP2 antibody (AA 1-156) (ABIN7161752)

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NCBP2 Antibody

Binding Specificity: AA 1-156

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Nuclear cap-binding protein subunit 2 protein (1-156AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:1000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Alternative Name: NCBP2

Background:

Background: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5\\\' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5\\\' end of mRNA and to mRNA export in a 5\\\' to 3\\\' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in \\\'failsafe\\\' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure.

Aliases: NCBP2 antibody, CBP20 antibody, PIG55 antibody, Nuclear cap-binding protein subunit 2 antibody, 20 kDa nuclear cap-binding protein antibody, Cell proliferation-inducing gene 55 protein antibody, NCBP 20 kDa subunit antibody, CBP20 antibody, NCBP-interacting protein 1 antibody, NIP1 antibody

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process