NME4 antibody (AA 1-187)

Catalog No. ABIN7162056

The Rabbit Polyclonal anti-NME4 antibody has been validated for IHC and ELISA. It is suitable to detect NME4 in samples from Human.

Quick Overview for NME4 antibody (AA 1-187) (ABIN7162056)

Target: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NME4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-NME4 Antibody

Binding Specificity: AA 1-187

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Nucleoside diphosphate kinase, mitochondrial protein (1-187AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NME4

Target: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Alternative Name: NME4

Background:

Background: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505). Binds to anionic phospholipids, predominantly to cardiolipin, the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663). Acts as mitochondria-specific NDK, its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663).

Aliases: metastatic inhibition factor NM23H4 antibody, mitochondrial antibody, NDK antibody, NDKM_HUMAN antibody, NDP kinase antibody, NDP kinase D antibody, NDP kinase, mitochondrial antibody, NDPK D antibody, NDPKD antibody, nm23 H4 antibody, nm23-H4 antibody, NM23D antibody, NM23H4 antibody, Nm23M4 antibody, NME/NM23 nucleoside diphosphate kinase 4 antibody, NME4 antibody, Non metastatic cells 4 protein expressed in antibody, Non metastatic protein 23, homolog 4 antibody, Nucleoside diphosphate kinase D antibody, Nucleoside diphosphate kinase, mitochondrial antibody, Nucleoside diphosphate kinase, mitochondrial antibody

UniProt: O00746

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process