TWF1 antibody (pTyr321)

Catalog No. ABIN712166

The Rabbit Polyclonal anti-TWF1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect TWF1 in samples from Human.

Quick Overview for TWF1 antibody (pTyr321) (ABIN712166)

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TWF1 Antibody

Binding Specificity: pTyr321

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TWF1

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: PTK9

Background:

Synonyms: PTK9 Tyr321, PTK9 Y321, p-PTK9 Tyr321, p-TWF1Y321, A6, A6 protein tyrosine kinase, Protein A6, Protein tyrosine kinase 9, PTK9, PTK9 protein tyrosine kinase 9, TWF 1, TWF1, Twinfilin 1, Twinfilin actin binding protein, homolog 1, Twinfilin, Twinfilin, actin-binding protein, homolog 1 Drosophila, Twinfilin, Drosophila, homolog of, 1, TWF1_HUMAN, Twinfilin-1.

Background: PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Gene ID: 5756

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location