TRIM25 antibody (pThr91) (Biotin)

Catalog No. ABIN712183

Product Details anti-TRIM25 Antibody (Biotin)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Binding Specificity: pThr91

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25 (TRIM25 Products)

Background:

Synonyms: TRIM25phospho T91_EFP, Estrogen responsive finger protein, RNF 147, RNF147, TRI25, TRIM 25, Tripartite mot containing 25, Tripartite mot containing protein 25, Z147, Zinc finger protein 147, Zinc finger protein 147 estrogen responsive finger protein, ZNF 147, ZNF147.

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Gene ID: 7706