MAGOH antibody (AA 1-146)

Catalog No. ABIN7165791

The Rabbit Polyclonal anti-MAGOH antibody has been validated for WB, ELISA, IHC and IP. It is suitable to detect MAGOH in samples from Human.

Quick Overview for MAGOH antibody (AA 1-146) (ABIN7165791)

Target: MAGOH (Mago-Nashi Homolog (MAGOH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGOH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-MAGOH Antibody

Binding Specificity: AA 1-146

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Protein mago nashi homolog protein (1-146AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MAGOH

Target: MAGOH (Mago-Nashi Homolog (MAGOH))

Alternative Name: MAGOH

Background:

Background: Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly.

Aliases: Mago nashi homolog proliferation associated (Drosophila) antibody, Mago nashi protein homolog antibody, magoh antibody, MAGOHA antibody, MGN_HUMAN antibody, Protein mago nashi homolog antibody

UniProt: P61326