QTRT1 antibody (Catalytic Subunit 1) (HRP)

Catalog No. ABIN7166921

This Rabbit Polyclonal antibody specifically detects QTRT1 in ELISA. It exhibits reactivity toward Human.

Quick Overview for QTRT1 antibody (Catalytic Subunit 1) (HRP) (ABIN7166921)

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This QTRT1 antibody is conjugated to HRP

Application: ELISA

Product Details anti-QTRT1 Antibody (HRP)

Binding Specificity: AA 211-403, Catalytic Subunit 1

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Queuine tRNA-ribosyltransferase catalytic subunit 1 protein (211-403AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for QTRT1

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Alternative Name: QTRT1

Background:

Background: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1\' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1\' of the ribose to form the product (By similarity).

Aliases: FP3235 antibody, Guanine insertion enzyme antibody, qtrt1 antibody, Queuine tRNA ribosyltransferase 1 antibody, Queuine tRNA ribosyltransferase antibody, Queuine tRNA-ribosyltransferase antibody, TGT antibody, TGT, 43 KD subunit antibody, TGT, catalytic subunit antibody, TGT_HUMAN antibody, TGUT antibody, tRNA guanine transglycosylase antibody, tRNA-guanine transglycosylase antibody

UniProt: Q9BXR0

Pathways: Ribonucleoside Biosynthetic Process