ROCK2 antibody (pTyr256)

Catalog No. ABIN713096

The Rabbit Polyclonal anti-ROCK2 antibody has been validated for ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and FACS. It is suitable to detect ROCK2 in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for ROCK2 antibody (pTyr256) (ABIN713096)

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Product Details anti-ROCK2 Antibody

Binding Specificity: pTyr256

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Tyr256

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ROCK2 antibody (ABIN713096)

Achiron, Feldman, Karmona, Avizemer, Barequet, Rosner, Knyazer, Bartov, Burgansky, Vishnevskia-Dai: "Prophylactic exposure of human corneal endothelial cells to Rho-associated kinase inhibitor reduced apoptosis rate after phacoemulsification: Ex vivo study." in: Journal of cataract and refractive surgery, Vol. 44, Issue 10, pp. 1261-1266, (2019) (PubMed).

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: ROCK2

Background:

Synonyms: ROCK2phospho Y256, p164 ROCK 2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho kinase 2, ROCK 2, Rock II, Rock2m, ROK alpha, ROKalpha, ROCK2_HUMAN.

Background: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Gene ID: 9475

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation