OTUB1 antibody (AA 2-201)

Catalog No. ABIN7174393

This anti-OTUB1 antibody is a Rabbit Polyclonal antibody detecting OTUB1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for OTUB1 antibody (AA 2-201) (ABIN7174393)

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-OTUB1 Antibody

Binding Specificity: AA 2-201

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Ubiquitin thioesterase OTUB1 protein (2-201AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for OTUB1

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Alternative Name: OTUB1

Background:

Background: Hydrolase that can specifically remove \\\'Lys-48\\\'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of \\\'Lys-48\\\'-linked polyubiquitin chains, but not \\\'Lys-63\\\'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to \\\'Lys-48\\\'-linked ubiquitin. Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of \\\'Lys-63\\\'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of \\\'Lys-63\\\'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved \\\'Lys48\\\'-linked di-ubiquitin chain.

Aliases: Deubiquitinating enzyme OTUB1 antibody, hOTU1 antibody, HSPC263 antibody, OTB1 antibody, OTU domain containing ubiquitin aldehyde binding protein 1 antibody, OTU domain, ubiquitin aldehyde binding 1 antibody, OTU domain-containing Ubal-binding protein 1 antibody, OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody, OTU-domain Ubal-binding 1 antibody, OTU1 antibody, Otub1 antibody, OTUB1_HUMAN antibody, Otubain 1 antibody, Otubain-1 antibody, Ubiquitin specific processing protease OTUB1 antibody, Ubiquitin thioesterase OTUB1 antibody, ubiquitin-specific protease otubain 1 antibody, Ubiquitin-specific-processing protease OTUB1 antibody

UniProt: Q96FW1