EGLN3 antibody (AA 2-239) (Biotin)

Catalog No. ABIN7177166

This Rabbit Polyclonal antibody specifically detects EGLN3 in ELISA. It exhibits reactivity toward Mouse.

Quick Overview for EGLN3 antibody (AA 2-239) (Biotin) (ABIN7177166)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-EGLN3 Antibody (Biotin)

Binding Specificity: AA 2-239

Cross-Reactivity: Mouse

Purification: >95%, Protein G purified

Immunogen: Recombinant Mouse Egl nine homolog 3 protein (2-239AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: Egln3

Background:

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.

Aliases: Egln3Egl nine homolog 3 antibody, EC 1.14.11.29 antibody, Hypoxia-inducible factor prolyl hydroxylase 3 antibody, HIF-PH3 antibody, HIF-prolyl hydroxylase 3 antibody, HPH-3 antibody, Prolyl hydroxylase domain-containing protein 3 antibody, PHD3 antibody, SM-20 antibody

UniProt: Q91UZ4

Pathways: Positive Regulation of Endopeptidase Activity