RUNX1 antibody (Ser435)

Catalog No. ABIN7179972

The Rabbit Polyclonal anti-RUNX1 antibody has been validated for WB and ELISA. It is suitable to detect RUNX1 in samples from Human.

Quick Overview for RUNX1 antibody (Ser435) (ABIN7179972)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX1 Antibody

Binding Specificity: Ser435

Purpose: RUNX1 (Ab-435) Antibody

Cross-Reactivity: Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background:

Background: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
Nucifora G., Blood 81:2728-2734(1993).

Aliases: Acute myeloid leukemia 1 antibody, Acute myeloid leukemia 1 protein antibody, alpha subunit core binding factor antibody, AML 1 antibody, AML1 antibody, AML1 EVI 1 antibody, AML1 EVI 1 fusion protein antibody, Aml1 oncogene antibody, AMLCR 1 antibody, AMLCR1 antibody, CBF alpha 2 antibody, CBF-alpha-2 antibody, CBFA 2 antibody, CBFA2 antibody, Core binding factor alpha 2 subunit antibody, Core binding factor runt domain alpha subunit 2 antibody, Core-binding factor subunit alpha-2 antibody, EVI 1 antibody, EVI1 antibody, HGNC antibody, Oncogene AML 1 antibody, Oncogene AML-1 antibody, OTTHUMP00000108696 antibody, OTTHUMP00000108697 antibody, OTTHUMP00000108699 antibody, OTTHUMP00000108700 antibody, OTTHUMP00000108702 antibody, PEA2 alpha B antibody, PEA2-alpha B antibody, PEBP2 alpha B antibody, PEBP2-alpha B antibody, PEBP2A2 antibody, PEBP2aB antibody, Polyomavirus enhancer binding protein 2 alpha B subunit antibody, Polyomavirus enhancer-binding protein 2 alpha B subunit antibody, Run1 antibody, Runt related transcription factor 1 antibody, Runt-related transcription factor 1 antibody, RUNX 1 antibody, Runx1 antibody, RUNX1_HUMAN antibody, SL3 3 enhancer factor 1 alpha B subunit antibody, SL3-3 enhancer factor 1 alpha B subunit antibody, SL3/AKV core binding factor alpha B subunit antibody, SL3/AKV core-binding factor alpha B subunit antibody

UniProt: Q01196