Splicing Factor 1 antibody (Ser82)

Catalog No. ABIN7180081

This anti-Splicing Factor 1 antibody is a Rabbit Polyclonal antibody detecting Splicing Factor 1 in WB, IHC, ELISA and IF. Suitable for Human.

Quick Overview for Splicing Factor 1 antibody (Ser82) (ABIN7180081)

Target: Splicing Factor 1 (SF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: Ser82

Cross-Reactivity: Human, Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human SF1 around the phosphorylation site of serine 82 (S-P-S(p)-P-E).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background:

Background: Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Susan Y. Park, Development, May 2005, 132: 2415 - 2423.
Dujin Zhou, Mol. Endocrinol., Jul 2000, 14: 986.
Dagmar Wilhelm, Genes & Dev., Jul 2002, 16: 1839.
Philipp Gut,Development, Oct 2005, 132: 4611 - 4619.

Aliases: SF1 antibody, ZFM1 antibody, ZNF162 antibody, Splicing factor 1 antibody, Mammalian branch point-binding protein antibody, BBP antibody, mBBP antibody, Transcription factor ZFM1 antibody, Zinc finger gene in MEN1 locus antibody, Zinc finger protein 162 antibody

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location