HDAC3 antibody (C-Term)

Catalog No. ABIN7180182

The Rabbit Polyclonal anti-HDAC3 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect HDAC3 in samples from Human.

Quick Overview for HDAC3 antibody (C-Term) (ABIN7180182)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HDAC3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from C-terminal of Human HDAC3.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background:

Background: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1, increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Xiaohong Zhang, Genes & Dev., Apr 2005, 19: 827 - 839.
Zhanguo Gao, J. Biol. Chem., Feb 2006, 281: 4540 - 4547. Andrew J. Wilson, J. Biol. Chem., May 2006, 281: 13548 - 13558.

Aliases: HD3 antibody, HDAC 3 antibody, HDAC3 antibody, HDAC3_HUMAN antibody, Histone deacetylase 3 antibody, RPD3 2 antibody, RPD3 antibody, RPD3-2 antibody, SMAP45 antibody

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development