MCM5 antibody

Catalog No. ABIN7181928

Product Details anti-MCM5 Antibody

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from Human MCM5 antibody.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5 (MCM5 Products)

Background:

Background: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity. Interacts with MCMBP.

Robert A. Sclafani, Genetics, May 2002, 161: 47.
Marylynn Snyder, PNAS, Oct 2005, 102: 14539 - 14544.
Christopher J. DaFonseca, PNAS, Mar 2001, 98: 3034.
Cathleen M. Lake, Genetics, Jun 2007, 10.1534

Aliases: CDC 46 antibody, CDC46 antibody, CDC46 homolog antibody, Cell division cycle 46 antibody, DNA replication licensing factor antibody, DNA replication licensing factor MCM 5 antibody, DNA replication licensing factor MCM5 antibody, MCM 5 antibody, MCM5 antibody, MCM5_HUMAN antibody, MGC5315 antibody, Minichromosome maintenance complex component 5 antibody, Minichromosome maintenance deficient (S. cerevisiae) 5 antibody, Minichromosome maintenance deficient 5 antibody, Minichromosome maintenance deficient protein 5 antibody, P1 CDC46 antibody, P1-CDC46 antibody

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA