DBF4B/DRF1 antibody (AA 41-140)

Catalog No. ABIN714161

The Rabbit Polyclonal anti-DBF4B/DRF1 antibody has been validated for ELISA, WB, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect DBF4B/DRF1 in samples from Human.

Quick Overview for DBF4B/DRF1 antibody (AA 41-140) (ABIN714161)

Target: DBF4B/DRF1 (DBF4B) (DBF4 Homolog B (DBF4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4B/DRF1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DBF4B/DRF1 Antibody

Binding Specificity: AA 41-140

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DBF4B/ASKL1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DBF4B/DRF1

Target: DBF4B/DRF1 (DBF4B) (DBF4 Homolog B (DBF4B))

Alternative Name: DBF4B

Background:

Synonyms: Activator of S phase kinase like protein 1, ASK like protein 1, ASKL1, chb, Chfon homolog B, DBF 4B, DBF4 homolog B S. cerevisiae, DBF4 homolog B, Dbf4 related factor 1, DRF1, Protein DBF4 homolog B, ZDBF1B, Zinc finger DBF type containing 1B, DBF4B_HUMAN.

Background: DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated.

Gene ID: 80174