Caspase 2 antibody (AA 251-350)

Catalog No. ABIN714971

The Rabbit Polyclonal anti-Caspase 2 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Caspase 2 in samples from Human.

Quick Overview for Caspase 2 antibody (AA 251-350) (ABIN714971)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Caspase 2 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: Caspase 2

Background:

Synonyms: Apoptosis related cysteine peptidase, CASP 2, Casp 2L Pro, CASP2, Caspase 2 apoptosis related cysteine protease, Caspase2, Developmentally down regulated 2, ICH 1, ICH 1 protease, ICH 1L, ICH 1L/1S, ICH1, ICH1 protease, ICH1L, ICH1L/1S, NEDD 2, NEDD2, NEDD2 apoptosis regulatory gene, Neural precursor cell expressed, Neural precursor cell expressed developmentally down regulated 2.

Background: Caspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.

Gene ID: 835

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway