Tumor Suppressor ARF (CDKN2A) (AA 1-156) antibody

Catalog No. ABIN7193535

The Mouse Monoclonal anti-Tumor Suppressor ARF antibody (Clone 4A10E1) (ABIN7193535) specifically detects Tumor Suppressor ARF in ELISA, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for Tumor Suppressor ARF (CDKN2A) (AA 1-156) antibody (ABIN7193535)

Target: Tumor Suppressor ARF (CDKN2A)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 4A10E1

Product Details

Binding Specificity: AA 1-156

Purpose: CDKN2A/P16 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human CDKN2A/P16 (AA: 1-156) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/500 - 1/2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details

Target: Tumor Suppressor ARF (CDKN2A)

Alternative Name: CDKN2A/P16

Background:

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene, this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Molecular Weight: 16.5 kDa

UniProt: P42771