MYLK antibody (AA 1375-1524)

Catalog No. ABIN7193606

The Mouse Monoclonal anti-MYLK antibody has been validated for WB, ELISA, IHC, ICC and FACS. It is suitable to detect MYLK in samples from Human and Mouse.

Quick Overview for MYLK antibody (AA 1375-1524) (ABIN7193606)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7F2B11

Product Details anti-MYLK Antibody

Binding Specificity: AA 1375-1524

Purpose: MYLK Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human MYLK (AA:1375-1524) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200-1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: MYLK

Background:

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

Molecular Weight: 210 kDa

Gene ID: 4638

UniProt: Q15746