WT1 antibody (AA 1-181)

Catalog No. ABIN7193614

This Mouse Monoclonal antibody specifically detects WT1 in IHC, FACS, WB, ELISA and ICC. It exhibits reactivity toward Human and Monkey.

Quick Overview for WT1 antibody (AA 1-181) (ABIN7193614)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 7F9E8

Product Details anti-WT1 Antibody

Binding Specificity: AA 1-181

Purpose: WT1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200-1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background:

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Molecular Weight: 49.2 kDa

UniProt: P19544

Pathways: Tube Formation