nectin-3 antibody (AA 431-549) (HRP)

Catalog No. ABIN715400

The Rabbit Polyclonal anti-nectin-3 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect nectin-3 in samples from Human and Mouse.

Quick Overview for nectin-3 antibody (AA 431-549) (HRP) (ABIN715400)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This nectin-3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-nectin-3 Antibody (HRP)

Binding Specificity: AA 431-549

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nectin 3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: Nectin 3

Background:

Synonyms: CD113, CDw113, DKFZP566B0846, FLJ90624, Nectin-3, Nectin3, Poliovirus receptor related 3, Poliovirus receptor related protein 3, Poliovirus receptor-related protein 3, PPR3, PRR3, PVRL 3, PVRL3, PVRL3_HUMAN, PVRR3.

Background: Nectins are immunoglobulin-like adhesion molecules that interact with afadin. Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin-catenin system in epithelial cells.The nectin/PRR-family consists of four members, nectin-1, -2, -3 and -4. All the members of the nectin family have two or three slice variants. Nectin 3 is mainly expressed in testis and placental tissues and interacts in vivo with both long and short isoforms of afadin.

Gene ID: 25945

Pathways: Cell-Cell Junction Organization