Sclerostin antibody (Biotin)

Catalog No. ABIN715588

This Rabbit Polyclonal antibody specifically detects Sclerostin in WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog and Pig.

Quick Overview for Sclerostin antibody (Biotin) (ABIN715588)

Target: Sclerostin (SOST)

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sclerostin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sclerostin Antibody (Biotin)

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Sclerostin

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Sclerostin

Target: Sclerostin (SOST)

Alternative Name: Sclerostin

Background:

Synonyms: BEER, Cortical hyperostosis with syndactyly, Sclerosteosis, Sclerostin, SOST, SOST_HUMAN, VBCH.

Background: Negative regulator of bone growth.Sclerostin (SOST) is a bone morphogenetic protein (BMP) antagonist, leading to the activation of BMP signaling. It negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs. It has been shown that Sclerostin binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. The noggin-sclerostin protein complex represents a novel mechanism for the fine-tuning of BMP activity in bone homeostasis. Evidence is accumulating that one of the important mechanisms of bone regulation by sclerostin is the modulation of Wnt/Beta-catenin signaling. Sclerostin also rapidly activated ERK-1/2 MAPK signaling, indicating the involvement of additional signaling pathways.

Molecular Weight: 24-28kDa

Gene ID: 50964