14-3-3 theta antibody

Catalog No. ABIN7213376

This Rabbit Polyclonal antibody specifically detects 14-3-3 theta in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 theta antibody (ABIN7213376)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 theta Antibody

Purpose: 14-3-3 θ/τ Polyclonal Antibody

Specificity: 14-3-3 θ/τ Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human 14-3-3 theta/tau around the non-phosphorylation site of S232

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 theta/tau

Background: Rabbit Anti-14-3-3 θ/τ Polyclonal Antibody,YWHAQ, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.,14-3-3 protein theta

Gene ID: 10971

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction