14-3-3 theta antibody (pSer232)

Catalog No. ABIN7223210

The Rabbit Polyclonal anti-14-3-3 theta antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect 14-3-3 theta in samples from Human, Mouse and Rat.

Quick Overview for 14-3-3 theta antibody (pSer232) (ABIN7223210)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 theta Antibody

Binding Specificity: pSer232

Purpose: 14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody

Specificity: Phospho-14-3-3 θ/τ (S232) Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein only when phosphorylated at S232.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human 14-3-3 theta/tau Phospho-Ser232

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 theta/tau

Background: Rabbit Anti-14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody,YWHAQ, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1,The protein encoded by PLN (phospholamban) is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in PLN are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.,14-3-3 protein theta

Gene ID: 5350

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction