AZI1 antibody (AA 280-360)

Catalog No. ABIN7213746

The Rabbit Polyclonal anti-AZI1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect AZI1 in samples from Human.

Quick Overview for AZI1 antibody (AA 280-360) (ABIN7213746)

Target: AZI1 (5-Azacytidine Induced 1 (AZI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AZI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AZI1 Antibody

Binding Specificity: AA 280-360

Purpose: AZI1 Polyclonal Antibody

Specificity: AZI1 Polyclonal Antibody detects endogenous levels of AZI1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human AZI1 at AA range: 280-360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for AZI1

Target: AZI1 (5-Azacytidine Induced 1 (AZI1))

Alternative Name: AZI1

Background: Rabbit Anti-AZI1 Polyclonal Antibody,AZI1, CEP131, KIAA1118, 5-azacytidine-induced protein 1, Centrosomal protein of 131 kDa, Cep131, Pre-acrosome localization protein 1,AZI1, also known as 5-azacytidine-induced protein 1, is a 1,083 amino acid protein that may play a role in spermatogenesis. AZI1 is most highly expressed in spinal cord, followed by testis, ovary, amygdala, cerebellum and thalamus. Low expression is present in other adult and fetal tissues and specific adult brain regions. AZl1 gene transcription begins in pachytene spermatocytes and expression of the gene is induced in cultivated fibroblasts on treatment with 5-azacytidine, which is known to lead to the demethylation of genomic DNA. The AZI1 gene is conserved in dog, cow, mouse, rat, chicken, and zebrafish, and exists as two alternatively spliced isoforms. AZI1 contains one IQ domain, and the gene that encodes it maps to human chromosome 17q25.3. Chromosome 17 makes up over 2.5 % of the human genome with about 81 million bases encoding over 1,200 genes. Alexander disease, Birt-Hogg-Dube syndrome and Canavan disease are also associated with chromosome 17.,5-azacytidine-induced protein 1

Gene ID: 22994

UniProt: Q9UPN4

Pathways: M Phase