Acetyl-CoA Carboxylase alpha antibody

Catalog No. ABIN7213446

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase alpha antibody is suitable to detect Acetyl-CoA Carboxylase alpha in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Acetyl-CoA Carboxylase alpha antibody (ABIN7213446)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Acetyl-CoA Carboxylase alpha Antibody

Purpose: Rabbit Anti-ACCα Polyclonal Antibody

Specificity: ACCα Polyclonal Antibody detects endogenous levels of ACCα protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ACCalpha around the non-phosphorylation site of S80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: ACCalpha

Background: ACACA, ACAC, ACC1, ACCA, Acetyl-CoA carboxylase 1, ACC1, ACC-alphaAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for ACACA.

Molecular Weight: 265kD

Gene ID: 31

UniProt: Q13085

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect