NCKAP1 antibody (AA 1001-1132) (HRP)

Catalog No. ABIN715745

The Rabbit Polyclonal anti-NCKAP1 antibody has been validated for WB and ELISA. It is suitable to detect NCKAP1 in samples from Mouse.

Quick Overview for NCKAP1 antibody (AA 1001-1132) (HRP) (ABIN715745)

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-NCKAP1 Antibody (HRP)

Binding Specificity: AA 1001-1132

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCKAP1L

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Alternative Name: NCKAP1

Background:

Synonyms: NCK associated protein 1, NCK associated protein 1, Membrane associated protein HEM2, Membrane-associated protein HEM-2, NAP 1, NAP1, NAP125, NCK associated protein, Nck-associated protein 1, Nckap1, NCKP1_HUMAN, p125Nap1.

Background: NAP125, also known as NCKAP1 (NCK-associated protein 1), p125Nap1 or membrane-associated protein HEM-2, is a 1,128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family. While widely expressed, NAP125 is found at highest levels in heart, brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, Abi-1 and CYFIP1. NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32.1 and mouse chromosome 2 C3. NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, suggesting a possible role in the disease.

Gene ID: 10787

UniProt: P55160

Pathways: RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis