Adducin alpha/beta antibody

Catalog No. ABIN7213490

This anti-not_set antibody is a Rabbit Polyclonal antibody detecting not_set in ELISA, IF, WB, IP and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Adducin alpha/beta antibody (ABIN7213490)

Target: Adducin alpha/beta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Purpose: Adducin α/β Polyclonal Antibody

Specificity: Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Adducin alpha/beta around the non-phosphorylation site of S726/713

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: Adducin alpha/beta

Background: Rabbit Anti-Adducin α/β Polyclonal Antibody,ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit beta,Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.,Alpha-adducin

Gene ID: 118, 119