Adducin alpha/beta (pSer713), (pSer726) antibody

Catalog No. ABIN7213298

The Rabbit Polyclonal anti-Adducin alpha/beta antibody is suitable to detect Adducin alpha/beta in samples from Human, Mouse and Rat. It has been validated for ELISA, IF, IHC and WB.

Quick Overview for Adducin alpha/beta (pSer713), (pSer726) antibody (ABIN7213298)

Target: Adducin alpha/beta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: pSer713, pSer726

Purpose: Rabbit Anti-Adducin α/β (phospho Ser726/713) Polyclonal Antibody

Specificity: Phospho-Adducin α/β (S726/713) Polyclonal Antibody detects endogenous levels of Adducin α/β protein only when phosphorylated at S726/713.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Adducin alpha/beta Phospho-Ser726/713

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Adducin alpha/beta

Background: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit betaAdducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Molecular Weight: 80kD

Gene ID: 118, 119

UniProt: P35611, P35612