AF4 antibody (AA 1-80)

Catalog No. ABIN7222492

This Rabbit Polyclonal antibody specifically detects AF4 in ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for AF4 antibody (AA 1-80) (ABIN7222492)

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AF4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AF4 Antibody

Binding Specificity: AA 1-80

Purpose: AF-4 Polyclonal Antibody

Specificity: AF-4 Polyclonal Antibody detects endogenous levels of AF-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human AF-4 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for AF4

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Alternative Name: AF-4

Background: Rabbit Anti-AF-4 Polyclonal Antibody,AFF1, AF4, FEL, MLLT2, PBM1, AF4/FMR2 family member 1, ALL1-fused gene from chromosome 4 protein, Protein AF-4, Protein FEL, Proto-oncogene AF4,AFF1 encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, AFF1has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.,AF4

Gene ID: 4299

UniProt: P51825