alpha 1 Sodium Potassium Atpase (pTyr260) antibody

Catalog No. ABIN7231444

The Rabbit Polyclonal anti-alpha 1 Sodium Potassium Atpase antibody is suitable to detect alpha 1 Sodium Potassium Atpase in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for alpha 1 Sodium Potassium Atpase (pTyr260) antibody (ABIN7231444)

Target: alpha 1 Sodium Potassium Atpase

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details

Binding Specificity: pTyr260

Purpose: Rabbit Anti-Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Sodium Potassium ATPase alpha-1 when Phospho occurs at Tyr260

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Antibody protein at AA range: 230-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: alpha 1 Sodium Potassium Atpase

Alternative Name: Sodium Potassium ATPase alpha-1

Background: Sodium/potassium-transporting ATPase subunit alpha-1, Na(+/K(+ ATPase alpha-1 subunit, Sodium pump subunit alpha-1The protein encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. ATP1A1 encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for ATP1A1.

Molecular Weight: 115kD

Gene ID: 476

UniProt: P05023