alpha-SMA (N-Term) antibody

Catalog No. ABIN7217658

This anti-alpha-SMA antibody is a Rabbit Polyclonal antibody detecting alpha-SMA in WB, IHC (p) and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for alpha-SMA (N-Term) antibody (ABIN7217658)

Target: alpha-SMA

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Product Details

Binding Specificity: N-Term

Purpose: α-SMA Polyclonal Antibody

Specificity: α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human alpha-SMA

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: alpha-SMA

Background: Rabbit Anti-α-SMA Polyclonal Antibody,ACTA2, ACTSA, ACTVS, GIG46, Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein,Actin aortic smooth muscle encoded by ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.,Actin aortic smooth muscle

Molecular Weight: observerd band 42kDa

Gene ID: 59

UniProt: P62736