AAK1 antibody (AA 240-320)

Catalog No. ABIN7213400

This anti-AAK1 antibody is a Rabbit Polyclonal antibody detecting AAK1 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for AAK1 antibody (AA 240-320) (ABIN7213400)

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AAK1 Antibody

Binding Specificity: AA 240-320

Purpose: AAK1 Polyclonal Antibody

Specificity: AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human AAK1 at AA range: 240-320

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for AAK1

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Alternative Name: AAK1

Background: Rabbit Anti-AAK1 Polyclonal Antibody,AAK1, KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1,Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 encodes a member (AP2 associated kinase 1) of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.,AP2-associated protein kinase 1

Gene ID: 22848

UniProt: Q2M2I8