BNIP3 antibody (Internal Region)

Catalog No. ABIN7217856

This anti-BNIP3 antibody is a Rabbit Polyclonal antibody detecting BNIP3 in WB, ELISA and IHC (p). Suitable for Human.

Quick Overview for BNIP3 antibody (Internal Region) (ABIN7217856)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BNIP3 Antibody

Binding Specificity: Internal Region

Purpose: BNIP-3 Polyclonal Antibody

Specificity: BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human BNIP-3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP-3

Background: Rabbit Anti-BNIP-3 Polyclonal Antibody,BNIP3, NIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3,BNIP3 is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The BCL2 interacting protein 3 interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.,BCL2

Molecular Weight: observerd band 22kDa

Gene ID: 664

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation