Beclin 1 antibody

Catalog No. ABIN7201595

This Mouse Monoclonal antibody specifically detects Beclin 1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Beclin 1 antibody (ABIN7201595)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C2

Product Details anti-Beclin 1 Antibody

Purpose: Beclin-1 Mouse Monoclonal Antibody (5C2)

Specificity: Beclin-1 protein detects endogenous levels of BECN1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Synthetic Peptide of Beclin-1 at AA range of 110-190

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1

Background: Mouse Anti-Beclin-1 Mouse Monoclonal Antibody (5C2),BECN1,BECN1 (beclin 1) encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.,BECN1

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy