BCAT1 antibody (Internal Region)

Catalog No. ABIN7218408

This anti-BCAT1 antibody is a Rabbit Polyclonal antibody detecting BCAT1 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for BCAT1 antibody (Internal Region) (ABIN7218408)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BCAT1 Antibody

Binding Specificity: Internal Region

Purpose: ECA39 Polyclonal Antibody

Specificity: ECA39 Polyclonal Antibody detects endogenous levels of ECA39 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human ECA39

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: ECA39

Background: Rabbit Anti-ECA39 Polyclonal Antibody,BCAT1, BCT1, ECA39, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), Protein ECA39,BCAT1 encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.,Branched-chain-amino-acid aminotransferase, cytosolic

Molecular Weight: observerd band 43kDa

Gene ID: 586

UniProt: P54687