BCR antibody (pTyr360)

Catalog No. ABIN7224058

The Rabbit Polyclonal anti-BCR antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect BCR in samples from Human, Mouse and Monkey.

Quick Overview for BCR antibody (pTyr360) (ABIN7224058)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BCR Antibody

Binding Specificity: pTyr360

Purpose: Bcr (phospho Tyr360) Polyclonal Antibody

Specificity: Phospho-Bcr (Y360) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y360.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Bcr Phospho-Tyr360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: Bcr

Background: Rabbit Anti-Bcr (phospho Tyr360) Polyclonal Antibody,BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26,A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene (BCR, RhoGEF and GTPase activating protein). The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for BCR.,Breakpoint cluster region protein

Gene ID: 613

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling