BCAS2 antibody (AA 150-230)

Catalog No. ABIN7213778

This anti-BCAS2 antibody is a Rabbit Polyclonal antibody detecting BCAS2 in WB and ELISA. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for BCAS2 antibody (AA 150-230) (ABIN7213778)

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BCAS2 Antibody

Binding Specificity: AA 150-230

Purpose: BCAS2 Polyclonal Antibody

Specificity: BCAS2 Polyclonal Antibody detects endogenous levels of BCAS2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human BCAS2 at AA range: 150-230

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BCAS2

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Alternative Name: BCAS2

Background: Rabbit Anti-BCAS2 Polyclonal Antibody,BCAS2, DAM1, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Spliceosome-associated protein SPF 27,BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53- or -mutant cells, this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.,Pre-mRNA-splicing factor SPF27

Gene ID: 10286

UniProt: O75934