Cadherin 5 antibody (Internal Region)

Catalog No. ABIN7201800

The Rabbit Polyclonal anti-Cadherin 5 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect Cadherin 5 in samples from Human, Mouse and Rat.

Quick Overview for Cadherin 5 antibody (Internal Region) (ABIN7201800)

Target: Cadherin 5 (CDH5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cadherin 5 Antibody

Binding Specificity: Internal Region

Purpose: VE-Cadherin Polyclonal Antibody

Specificity: VE-Cadherin Polyclonal Antibody detects endogenous levels of VE-Cadherin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human VE-Cadherin

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:50-1:300), ELISA (1:10000-1:20000). Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: VE-Cadherin

Background: Rabbit Anti-VE-Cadherin Polyclonal Antibody,CDH5, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144,CDH5 encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. CDH5 is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.,Cadherin-5

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2